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The master reaction mix contained 5 ml reaction buffer (0.1 M Tris-HCl, pH8.6), 1 ml cosolvent (3% Triton X-100), 2 ml chromogenic reagent (120 mg Nitrotetrazolium blue chloride, 4 mg Phenazine methosulfate and 250 mg NAD + dissolved in 50 ml sterilized water) and 130U LDH were prepared

(Figure 4) Autophagy has been shown to be involved in lipid metabolism (Kovsan et al., 2010)

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