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After thorough washing with PBS, CoraLite 594 Goat Anti-Rabbit IgG (Proteintech, China) was applied and incubated for 1 h at room temperature

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9) Impute folds test data: Within each fold, the test data (20% of the data) underwent imputation to deal with missing data using the same method for imputation of folds training data

The EGL-9 protein uses molecular oxygen to hydroxylate the hypoxia-inducing factor, or HIF, inhibiting HIF transcriptional activity