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Most research protocols cap single shots around 300 mcg because GH release flattens out beyond that point more dose does not equal more GH pulse

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In diazepam+bpc 157 10 microg- conditioned mice, a residual anticonvulsive activity (i.e., longer latency to isoniazid convulsion) was noted at 6 h post-conditioning, whereas shorter preconvulsive latencies appeared only at 72 h-post-conditioning period