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Because copper is a required cofactor for numerous enzymes involved in cellular metabolism and antioxidant activity, researchers studying GHK-Cus copper-binding properties often position it as a tool for understanding copper-dependent signaling pathways generally, not just those specific to skin or tissue repair

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Luminescence measurements were performed using 475/30 nm and 535/30 nm filters with baseline measurements taken for ~3 min before addition of vehicle or peptide and reading resumed for a further 10 min