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Am J Physiol Lung Cell Mol Physiol 283: 766-776

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The protein stock solution was diluted 17-fold into D 2 O-based buffer (20 mM HEPES pH 7.5, 100 mM NaCl) to achieve a final D 2 O fraction of 94% and to maintain an optimal dynamic range of deuterium uptake, thereby improving the reproducibility of HDX-MS measurements