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The rationale traces back further, to Kraus and colleagues in 2014 ( Nature ), who knocked down NNMT genetically in mice and protected them from diet-induced obesity

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In mouse studies with diet-induced obesity, this led to real weight loss, smaller fat cells, less body fat, lower cholesterol, all without cutting food intake or causing obvious side effects

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The number of articles after applying these filters were recorded under Filters added. For each review (1a-b, 2a-b, 3a-d), results from all databases were consolidated, duplicates were removed, and abstracts were screened based on the respective inclusion and exclusion criteria