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Isolated microvessels from individual mice were plated in Endobasal Medium (Promocell) with 0.4% puromycin for positive selection on coverslips or polyester transwell inserts (Corning)

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Here are the steps for this stretch: Start on a mat on your hands and knees

Enhances Cellular Migration and Cytoskeletal Remodeling: TB-500 regulates actin polymerization and cytoskeletal dynamics, enabling efficient cell motility and structural repair during tissue regeneration 2