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Description

It is believed that the enzyme l-GalLDH may influence the respiratory process (Millar et al., 2003), bypassing the phosphorylating respiratory Complexes I and III ( Figure 4B )

The pellet containing the proteins was reconstituted with 100 mM Tris (pH 8.5) and 2% sodium deoxycholate and alkylated by adding 5 mM TCEP and 30 mM chloroacetamide at 56 C for 10 min