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Time heat and cold modalities so they complement rather than blunt training adaptations

The canonical PINK1Parkin-mediated mitophagy enables the recognition and ubiquitination of damaged mitochondria, facilitating their autophagic clearance, while the receptor-mediated pathways involving BNIP3, NIX, and FUNDC1 provide additional layers of regulation, particularly under stress conditions relevant to aging and NDDs [59,60,61,62]

Wang from the Institute of Biophysics for sharing human BJ fibroblast cells and the assistance with cell culture

Stampoulis, D., Sinha, S

Briefly, cortices were digested with 1 mg/ml collagenase/dispase and 2.5 g/ml DNAse (Roche) in dissection buffer (HBSS, 10 mM HEPES, 0.5% BSA, 5000 U/ml penicillin/streptomycin) for 45 min at 37 C