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Avoid freezing the mixed solution and do not subject it to repeated freezethaw cycles

Drug solutions, typically 5 mg/ml in HPLC-grade water (except for Dihexa, prepared in DMSO), were added to rat serum, and the mixture was incubated at 37C

Given their contribution to the maintenance of stem-like features of GIC, we first evaluated how these two pathways alone and their balance influence the expression of stem-like markers and self-renewal capacity

Gmez-Palacio-Schjetnan A, Escobar ML

46 Factors affecting amino acid uptake include plasma concentration, affinity of the transport system for a particular amino acid, and competition among amino acids for a particular transport system