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Notably, only dermal papilla cells exhibited a germ-like structure, whereas spherical fibroblasts and keratinocytes elongated in a unidirectional manner

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Purified proteins were re-suspended in buffer (250 mM HEPES, 8 M urea, 0.1% SDS, pH 7.5) and replaced with buffer I (25 mM HEPES, 1 M urea, pH 7.5) three times in an ultrafiltration centrifugal tube (Merck, Amicon Ultra-0.5 mL, 10 KDa)

Extracts were cleared by centrifugation at 15,000 g and 4 C for 15 min, boiled at 95 C for 5 min and finally analyzed by SDS-PAGE and Western blots using antibodies against GFP, -tubulin as loading control, and HA-tag when required

The median CA 19-9 level, a prognostic marker commonly used to reflect disease burden and PDAC aggressiveness, was similar between groups at diagnosis, although it trended towards a higher value in the high glucose group (2439.5 U/mL high glucose vs 1294.5 U/mL normal glucose, P =0.224)