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Predrag Sikiric, isolated and synthesized BPC-157 at the University of Zagreb as part of their research

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and Bobby Harshbarger, a Tennessee state senator and pharmacist at his family's compounding pharmacy, which sells longevity, weight-loss, and pain peptides

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Because so much of GHK-Cus activity is copper-mediated, it is difficult in some experiments to separate specific tripeptide signaling from generic copper effects a point made concrete by the finding that the MMP-2 effect was reproduced by copper ions alone while the collagen-synthesis effect required the intact peptide