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Briefly, 40-m coronal sections were rinsed with 0.05 M Tris-buffered saline (TBS) for 10 min and incubated in TBS containing 0.5% (v/v) Triton-X 100 (TBST) for 1 h

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45 Exclusion criteria included any contraindication to MRI, history of epilepsy, stroke, brain surgery, or structural brain disease, pregnancy, history of sulfur sensitivity, ongoing asthma or drug dependence, ongoing chronic diseases, history of mental illness, or acute infection during the prior 30 days

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Scenario 1: slightly past expiration, properly stored If your tirzepatide is a few days past the printed expiration date and has been stored correctly at 2 to 8 degrees Celsius the entire time, it has likely not undergone dramatic degradation