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Primary patient-derived AML cells were isolated using Ficoll gradients and were cultured in IMDM (Thermo Fisher Scientific, USA) supplemented with 15% BIT 9500 Serum Substitute (STEMCELL Technologies, Canada), 100 ng/ml stem cell factor (SCF) (ImmunoTools, Germany), 50 ng/ml FLT3L (ImmunoTools, Germany), 20 ng/ml interleukin-3 (IL-3) (ImmunoTools, Germany), 20 ng/ml G-CSF (ImmunoTools, Germany), 0.1 mM 2-mercaptoethanol (Gibco, USA), 50 g/ml gentamicin (Thermo Fisher Scientific, USA), 10 g/ml ciproflaxin (Thermo Fisher Scientific, USA), 500 nM SR1 (APExBIO, USA) and 1 M UM279 (APExBIO, USA) at 37 C, 5% CO 2 , and 95% humidity

doi: 10.1038/s41419-020-2317-3 9 FengH.LiuQ.DengZ.LiH.ZhangH.SongJ.et al

Cannabinoids inhibit the synaptic uptake of adenosine and dopamine in the rat and mouse striatum

Symptoms and Detox Reactions: What to Expect After Your IV Drip While IV therapy for hair growth is generally well-tolerated and side effects are minimal, its important to be aware of potential symptoms or detox reactions that a small percentage of individuals might experience, especially after their first session or with drips containing powerful antioxidants like Glutathione