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Biofilms were visualised by Confocal Laser Scanning Microscopy (CSLM) (Olympus FV1200, Australia) with Ex/Em 473/559 nm and Ex/Em 500/637 nm for Syto 9 (green for live cells) and PI (red for damaged and dead cells) staining, respectively

Two-dimensional nanomaterial Two-dimensional nanomaterials, including graphene and its derivatives, transition metal disulfide nanosheets (TMDs), black phosphorus, transition metal oxides (TMOs), and silicene nanosheets, typically possess extensive specific surface areas and robust chemical stability

The observed increased expression of several proteins involved in carbohydrate catabolism (total 16), including the TCA cycle adds support to the idea of increased energy requirements during nicotine exposure and detoxification