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When to use SUMO: When you need a native N-terminus (structural biology, functional studies) When tag size matters but you still want solubility enhancement When clean, efficient cleavage is critical Excellent for proteins destined for crystallization or NMR When NOT to use SUMO: In eukaryotic expression systems (host SUMO proteases will cleave prematurely) When the N-terminus is buried or critical for folding (Ulp1 won't access it) GST (Glutathione S-Transferase) Size: ~26 kDa (211 residues) Mechanism: GST provides moderate solubility enhancement and convenient purification via glutathione-Sepharose

Nrf2 regulates important genes such as heme-oxygenase 1(HO-1), glutathione S-transferase (GST), glutathione reductase (GR), glutamate-cysteine ligase subunits (GCLc and GCLm), NAD(P)H quinone oxidoreductase-1 (NQO1), and thioredoxins (TrxR1), among others

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Beyond promoting detoxification via bile excretion, TUDCA offers cell-protective effects: it stabilizes mitochondrial membranes, reduces oxidative stress and endoplasmic-reticulum (ER) stress, and blocks apoptosis (cell death) in hepatocytes