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This system uses selective digestion of sample DNA with methylation-sensitive and methylation-dependent restriction enzymes, followed by quantification of the remaining DNA using real-time PCR

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Hippocampal neurons transfected with mRFP-P-actin alone or with shMet to knock down the c-Met receptor were stimulated with HGF (10 ng ml), Dihexa (10-12 M) or Nlel-AnglV (10-12 M) for 48 hours