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CAT#: Cytiva Cytiva Glutathione Sepharose 4B Chromatography Resin, 300 mL/Unit Chromatography Resin, 10 mL/Unit Chromatography Resin, 100 mL/Unit 3 product variations: View Options Product Overview High capacity, single-step glutathione Stransferase (GST) tagged protein purification Supplied as preswollen bulk (loose) resin Chemically stable with all commonly used aqueous buffers Suitable for batch GST tagged protein purification and scaleup Used in GST pulldown assays to study proteinprotein interactions Product Overview High capacity, single-step glutathione Stransferase (GST) tagged protein purification Supplied as preswollen bulk (loose) resin Chemically stable with all commonly used aqueous buffers Suitable for batch GST tagged protein purification and scaleup Used in GST pulldown assays to study proteinprotein interactions Product Overview High capacity, single-step glutathione Stransferase (GST) tagged protein purification Supplied as preswollen bulk (loose) resin Chemically stable with all commonly used aqueous buffers Suitable for batch GST tagged protein purification and scaleup Used in GST pulldown assays to study proteinprotein interactions Technical Documents Technical Documents Detailed Description Glutathione Sepharose 4B is an affinity chromatography resin for batch purification of GST-tagged proteins and when high binding capacity is required

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Although xCT expression is confined to the plasma membrane in normal tissues (36,39), it is upregulated in certain types of cancer, such as colorectal cancer, malignant glioma and non-small cell lung cancer, which increases cystine uptake (4042)

Following the injection, imaging was performed using an IVIS Spectrum system (PerkinElmer) at various time points: 1 h, 4 h, 7 h, 12 h, 24 h, 36 h, 48 h, and 60 h post-injection, with an excitation wavelength of 780 nm and a fluorescence emission wavelength of 840 nm