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The Abl KD and SH2-KD proteins in pET21d vectors containing a C-terminal His 6 tag were each co-expressed with YopH phosphatase in TB medium and purification was performed as described previously using nickel-affinity chromatography (His-Trap FF crude, Cytiva) and anion exchange chromatography (Mono Q 5/50 GL, Cytiva) on an kta Avant system (Cytiva) 13,52

J Neurol Sci 167:121126 Guiloff RJ, Emami JM, During MJ, Freese A, Plaitakis A, Mandeli J, Smith J, Yahr MD, Shashidharan P, Malessa S, Leigh N, Hornykiewicz O (1988) Branched-chain aminoacids in amyotrophic lateral sclerosis

Imaging 54 , 265270 (2018)

Saito ER, Warren CE, Hanegan CM, Larsen JG, du Randt JD, Cannon M, Saito JY, Campbell RJ, Kemberling CM, Miller GS, Edwards JG, Bikman BT (2022) A novel ketone-supplemented diet improves recognition memory and hippocampal mitochondrial efficiency in healthy adult mice