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Methods Experimental protocols Sixty C57BL/6J male mice (1820 g, 810 weeks, Changsheng Biotechnology Company, Liaoning, China) were housed and fed at the First Hospital of China Medical University, the Institute of Respiratory Disease, under quiet and controlled specific pathogen-free conditions

Sirtuin activation Sirtuins (SIRT1-7) are NAD+-dependent enzymes that regulate gene expression, mitochondrial biogenesis, inflammation, and stress responses