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Immunocytochemistry For nuclear co-localization experiments, SH-SY5Y neuronal cells (ECACC

FDH Formate dehydrogenase is a mitochondrial-localized NAD-requiring enzyme while the HCOOH is getting into the mitochondrial, FDH will oxidize the formic acid into CO2, and reduce NAD+ to NADH with a high degree of specificity

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The relatively weak selectivity of the Ni-NTA matrix for the common 6histidine tag endows one-step immobilized metal affinity chromatography (IMAC) purification with a desirable protein quality only from pure tissue culture supernatants