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Post-centrifugation at 350 g for 5 minutes, cells were lysed in 3 ml ACK buffer for 5 minutes, then diluted to 35 ml with RPMI with HEPES buffer and without antibiotics (RPMI-MGIA) and centrifuged again

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There are approximately 470 institutes in the UK across all industry sectors, with a collective membership of circa 4 million professionals, and they all expect their members to undertake CPD