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The liver is responsible for filtering toxins, heavy metals, alcohol, and medications from the bloodstream, and glutathione acts as a shield binding to these harmful substances and facilitating their elimination

Further, the serum ALT activity was significantly lower ( p = 0.046) in T1_HS and T2_HS as compared to CON_HS, wherein CON had an intermediate ALT activity

This reagent reacts with the thiol groups of GSH which convert 2-nitro-5-thiobenzoate (NTB ) to NTB 2 , thereby producing a yellow color, which can subsequently be quantified ( Glutathione Peroxidase GPx is measured by adding glutathione reductase (GR) to the sample after which nicotinamide adenine dinucleotide phosphate (NADPH) is transformed to a reduced state, allowing a decrease in absorbance to be subsequently measured ( Lipid Peroxidation OS leads to cellular injury via several mechanisms, with lipid peroxidation being a prominent one

Immunohistochemistry assay On the 17th day, the mice were euthanized, and both tumor tissues and major organs were collected

doi: 10.1136/gutjnl-2017-315542 7