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Amplified fragments were purified with AMPure XP Beads (Beckmann Coulter Genomics), combined and analyzed by 2 300 bp paired-end sequencing using an Illumina MiSeq device

Wauer T, Komander D

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Abstract Polycystic ovarian syndrome (PCOS) is a common heterogeneous reproductive endocrine metabolic disorder in women of reproductive age characterized by clinical and biochemical hyperandrogenemia, ovulation disorders, and polycystic ovarian morphology

All drugs were prepared in normal saline and stored as frozen stock solutions, with aliquots thawed immediately prior to use