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Treatment with the transition-metal chelating agents deferoxamine and trientine, which can prevent auto-oxidation, corrected nerve conduction and blood flow changes that occurred 2 months after the induction of diabetes in this model [28]

Researchers do this to equalise pressure and make drawing easier, but it introduces airborne contaminants and establishes a positive pressure differential that forces solution back through the needle tract on every subsequent draw

10L of the supernatant was reserved to determine protein concentration, and Laemmli dye was added to 150L of supernatant

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