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The resulting cDNA was used as a template for quantitative PCR (qPCR) using the SYBR Green qPCR low ROX 2 Mastermix (Agilent) to quantify the expression of the various midgut-specific alkaline phosphatase genes normalized to rp49 as an internal control

doi: 10.1111/ijfs.2017.52.issue-1 46 ShujaatN.AhmadY.IkramM.ShaheenS.IshaqM.HameedT.et al

It not only supports genomic stability but may also influence the expression of genes involved in cellular stress responses

Hereafter, the medium was removed, tissue 4% PFA-fixed for 20 min, washed twice with excess PBS and permeabilized by incubating in PBS0.1% Triton X-1002% BSA for 1 h