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Gene expression analysis by reverse transcriptase-quantitative real-time PCR (RT-qPCR) After the homogenization step by using a TissueLyser II (Qiagen, Hilden, Germany) in the gastric tissue, total RNA was isolated using a commercial kit (12183018A PureLink RNA Mini Kit, Thermo Fisher) in accordance with the manufacturers recommendations

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[DOI] [PMC free article] [PubMed] [Google Scholar] [87].Raskob GE, Angchaisuksiri P, Blanco AN, Buller H, Gallus A, Hunt BJ, et al