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The reaction volume of 20 l mix of 10 L SYBR Green, 0.6 L of both forward and reverse primer, 1 L of template cDNA, and the total volume of 20 L adjusted with nuclease-free water

Kinetic analyses of the fecal excretion of radioiodine in euthyroid subjects also supports a direct blood-to-intestine excretion route for iodide (Hays 1993)