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The buffer containing nuclei was transferred to a clean microcentrifuge tubes for each treatment and the tubes were centrifuged at 12,000 rpm for 15 min

doi: 10.1371/journal.ppat.1001209 75 KaminskyL

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Most catalases are haemin-containing enzymes (i.e., their active sites consist of a ferric Fe 3+ ion complexed within a planar cyclic tetradentate porphyrin ligand [Milgrom, 1997]) made up of tetrameric protein subunits (see Figure 2)