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In vitro kinase/phosphatase assay For phosphorylated GST-GSK3 protein, purified GST-GSK3 protein (2 g) was incubated with 0.5 g recombinant AKT protein (Sigma, #14-276-M) in kinase buffer (100 mM MOPS/NaOH (pH 7.2), 20 mM MgCl 2 , 1 mM DTT, 1 mM EDTA, and 1 mM PMSF) with 1 mM adenosine 5-triphosphate (ATP) (Sigma-Aldrich) at 37 C for 1 h

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Membrane Phospholipid Peroxidation Promotes Loss of Dopaminergic Neurons in Psychological Stress-Induced Parkinsons Disease Susceptibility

Elemental quantification and image analysis Analysis of elemental XRF maps was performed using a combination of tools native to GeoPIXE and ImageJ 57