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Evolution of inflammatory diseases

After 90 min of basal sampling, the clamp began at t = 0 with a continuous infusion of human insulin (2.5 mU/kg/min for mice fed chow diet & 4 mU/kg/min for mice fed high-fat diet) after a bolus and the infusion of [3- 3 H] glucose was increased to 0.1 Ci/min for the rest of the experiment