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Serum ZAG concentration declines progressively with age, showing an inverse correlation with fasting blood glucose and triglyceride levels, while higher body mass index (BMI) is also negatively associated with ZAG levels [450]

Every subsequent draw from the same vial occurs from an increasingly contaminated solution

Absorption problems Even with adequate intake, absorption can be reduced by: Certain gastrointestinal conditions affecting the stomach or small intestine Some medications Age-related reduction in stomach acid (which can affect absorption) Autoimmune gastritis (often historically referred to as pernicious anaemia) This is a well-recognised cause of B12 deficiency and may require ongoing management

In vitro , specificity data are incomplete regarding ascorbate as the required electron donor (as opposed to other reducing agents)

All statistical analyses were conducted using GraphPad Prism (version 9) software (GraphPad Software Inc., San Diego, CA)