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Consequently, GPX4 inhibitors behave as a alternative class of senolytic drugs that kill senescent cells by ferroptosis and can be used to treat senescence-associated diseases such as cancer

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The genomic region flanking the CRISPR target site for each gene was PCR amplified (primers listed in Table E), and products were purified using QiaQuick Spin Column (Qiagen) following the manufacturer's protocol

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