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Afterwards, non-adherent cells were washed away, and wells were incubated with HRP anti-His 6 antibody

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Additionally, to avoid biases introduced by the enrichment step (which can alter the sequence distribution in the library), a control sample reverse-transcribed with SSIV (which does not induce deletions) was used for background correction when enrichment was performed

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This is where the quality of the synthesis process becomes paramount