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Results & discussion Method validation The HPLC method was validated as to specificity, linearity, sensitivity, accuracy, precision (repeatability and reproducibility), LOD, LOQ, and stability as per the ICH [50]ICH Q2 (R1), Validation of analytical procedures: text and methodology, in Proceedings of the International Conference on Harmonization, Geneva, Switzerland, 2005

The study comprised two cohorts: Cohort 1 included participants with negative SARS-CoV-2 RT-PCR test results on day 1, and Cohort 2 included participants with positive SARS-CoV-2 RT-PCR test results