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Cells were permeabilized using digitonin (1 g per 10 6 platelets or 6 g per 10 6 PBMCs), and simultaneously, the N-pathway substrates (or CI substrates) malate and pyruvate were added at saturating concentrations (5 mM each)

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DPPH, 2,2-diphenyl-1-picrylhydrazyl expresses percentage inhibition of the DPPH radical-scavenging activity NSO, Nigella sativa oil

Fluorescence was recorded at 545 nm emission after 520 nm excitation for 45 minutes using a Victor3 fluorescence plate reader (PerkinElmer, USA)