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To evaluate cross-reactivity, we performed flow cytometry-based binding tests of the obtained mAbs with E proteins from other alphaviruses

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Moreover, it is important to underline the contradictions and the controversial outcomes found in the literature

MT3-WT, GS-MT3, and G-MT3 obtained from SUMO-LPETG-MT3 were also purified using size exclusion chromatography (SEC) in 10 mM HCl to avoid protein oxidation during separation (Additional file 1: Fig