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Chromatographic separation is performed on an Agilent series 1100 HPLC device with a Zorbax Eclipse XDB-C18 column and fluorometric detector

In addition, ACSL4 inhibition may ameliorate renal injury by decreasing the levels of lipid peroxidation products and inhibiting ferroptosis (123)

For selectivity studies, recombinant GSTO1 (1 M), PDI (1 M), BSA (1 M) or GRP78 (1 M) in reaction buffer were incubated with C1-27 or DMSO for 30 min at 37 C, followed by incubation with CMFDA (500 nM), BODIPY- C1-27A (1 M) or DMSO for 30 min