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Genomic analysis of the TRIM family reveals two groups of genes with distinct evolutionary properties

Determination of optimal drug concentrations for CRISPR screen HEK293 cells were seeded in 6-well plates at a density optimized to remain below 90% confluency after 72 h of treatment with minimal concentrations of drugs either inhibiting mitochondrial translation (chloramphenicol) or inducing mitochondrial mistranslation (actinonin)

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Wrinkle depth assessment by means of DPI resulted in a statistically significant improvement only in the periocular area in the MCN group, without any significant change in the placebo group