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The cultures were freeze-thawed three times, cell debris removed by low speed centrifugation and supernatants titrated by plaque assay on Vero cell monolayers

Mitotic index (Figure 3b) and the percentage of phosphorylated histone H3-positive hepatocytes (Figures 3c and d) further evidenced a significant decrease in the proliferation rate in the Gstp1/2 / livers compared with the WT livers