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During milk kefir fermentation, yeast will metabolise lactose into fermentation metabolites, mainly lactic acid, ethanol, carbon dioxide and acetic acid (Ganatsios et al., 2021

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sc-13324, Santa Cruz), and -actin (1:10000, Sigma-Aldrich), washed with PBST and then probed with their respective secondary antibodies conjugated to horseradish peroxidase for 1 h at room temperature