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pOPINK-Cezanne (OTU, aa 53446) was a gift from David Komander (Addgene plasmid #61581

To determine the robustness of the screen the statistical parameter, Z-prime (Z) was determined (25) and at 40 hours of growth the values obtained were very significant (for Screen I =0.95 and for Screen II = 0.91) which suggested that the screens we developed were robust and would be compliant for a high throughput setting (Figure S6)

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In todays fast-paced world, where energy demands are high and time seems limited, many individuals turn to stimulants like coffee or energy drinks to power through their days

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