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Additionally, ROS-modulated gene expression increases endothelial permeability, inflammation, and vascular disease by affecting T-cell polarization and cytokine secretion [34]

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The answer to the first question is that he first performed a preliminary study with a wider range of doses, reported in the same paper

Our findings (Shen et al., 2022) indicate that Mlb treatment lessens the severity of CI-AKI by suppressing ROS generation, apoptosis, and mitochondrial damage by modulating Drp1 levels

Similarly, GKT137831 effectively inhibited oxidative stress triggered by hyperglycemic conditions in human aortic endothelial cells (HAECs) in vitro [184]