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After the imaging, the cells were trypsinised, re-suspended in the complete media, and relative number of living cells were counted by using Countess II after staining with trypan blue

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This powder often looks like a small cake or a light dusting at the bottom of the glass

These results suggest that reduced homocysteine causes protein thiol homocysteinylation that may induce smooth muscle cell proliferation by binding to a redox receptor and that may exacerbate atherosclerotic lesion formation by inducing collagen expression (Tyagi, 1998)