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Electroporation buffer and unloaded RFP were removed by ultrafiltration via an Amicon centrifugal filter (Millipore, UFC510096, 100 kDa molecular weight cutoff) followed by three washes with DPBS buffer (10 mM, pH 7.4, 13,000 g) at 4 C

50,51 They may be less responsive to folic acid therapy and may require higher supplemental folate doses

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Nitric oxide synthase 2 (NOS2) is another major catabolic enzyme for L-Arg metabolism in MDSCs (105)