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Differentiation of 3T3-L1 pre-adipocytes 3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work.[18] Briefly, standard culture media was supplemented with scheduled addition of adipogenic agents [3-isobutyl-1methyl xanthine (IBMX), Sigma Aldrich

AOD 9604 does not stimulate muscle growth, but it does not promote muscle breakdown either

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High visceral fat is strongly associated with: Type 2 diabetes Cardiovascular disease Fatty liver disease Hypertension Cognitive decline Increased all-cause mortality Even individuals with a normal body mass index may carry excess visceral fat, a phenomenon often referred to as TOFI (thin outside, fat inside)

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