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The 2 Ct method was employed to calculate the relative amount of the target gene, where Ct = (Ct Target gene Ct RPLP0 ) GDC test (Ct Target gene Ct RPLP0 ) Control

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Disclosure of interest: RJvO and WHvZ report financial support for the current manuscript from the Collaboration for New Treatments of Acute Stroke (CONTRAST) consortium (which was financed for the current study by the Netherlands Cardiovascular Research Initiative, an initiative of the Dutch Heart Foundation, the Netherlands Brain Foundation, Stryker, Medtronic, and Cerenovus), all paid to their institution

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