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Epidermal H(2)O(2) accumulation alters tetrahydrobiopterin (6BH4) recycling in vitiligo: identification of a general mechanism in regulation of all 6BH4-dependent processes

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Research Area Workflow Stages Quality Statement: PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.

Central to all protocols in the preparation of PCLS is that the freshly retrieved whole lungs or individual lobes are infused with warm, low-melting agarose dissolved in buffer or tissue culture medium, at a concentration ranging from 0.5% to 3% (38) ( Figure 1 )

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