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These findings suggest that high baseline autophagic activity, particularly when coupled with elevated checkpoint expression, could serve as a biomarker for identifying patients who may benefit from combining autophagy inhibitors with immunotherapy, a hypothesis awaiting robust clinical validation

Briefly, retinas were dissected from eyes fixed with 4% paraformaldehyde in PBS for 1 h at room temperature and then postfixed with the same fixative solution overnight at 4 C