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Seeds were surface sterilized with bleach (3% sodium hypochlorite) for 8 min before being plated on half-strength sucrose-free Murashige and Skoog (1/2 MS0) media supplemented with Gamborgs vitamins (Caisson Laboratories, North Logan, UT), 0.5 mM MES (pH 5.7), and 0.8% (w/v) agar (Caisson Laboratories, North Logan, UT)

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It demonstrates that the disruption of thiometabolism and disulfide stress play critical roles in CNS diseases, which are associated with the potential role of disulfidptosis

TBARS levels increased significantly in the brain of animals in the TSE and TSE + P groups in comparison to the control